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Each line represents the mean and standard error bars of 7 rats

Your coordination diminishes, increasing the risk of accidents

The stimulation index was calculated by dividing the test condition by the media alone control (baseline)

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Body weight, surface temperature and food consumption were measured weekly, and animals were observed twice daily for signs of clinical toxicity and mortality

This can lead to inconclusive or misleading results, forcing you to repeat experiments and consume more of your valuable time and budget