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E2E1-I53-50A engaged several HCV bNAbs targeting different epitopes, including AP33 (antigenic site 412 (AS412)/domain E), AR3B (antigenic region 3 (AR3)/domain B), AT1211 (domain C), HC84.26 (domain D), all located on E2, as well as IGH505 which targets an epitope on E1, but not AR4A and AT1618, which target conformational epitopes that require natively folded E1E2 (Fig

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