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[6] During muscle contraction: H 2 O + ATP H + + ADP + P i (Mg 2+ assisted, utilization of ATP for muscle contraction by ATPase) H + + ADP + CP ATP + Creatine (Mg 2+ assisted, catalyzed by creatine kinase, ATP is used again in the above reaction for continued muscle contraction) 2 ADP ATP + AMP (catalyzed by adenylate kinase/myokinase when CP is depleted, ATP is again used for muscle contraction) Muscle at rest: ATP + Creatine H + + ADP + CP (Mg 2+ assisted, catalyzed by creatine kinase) ADP + P i ATP (during anaerobic glycolysis and oxidative phosphorylation) AMP can dephosphorylate to adenosine and diffuse out of the cell

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The change in m was observed under a Nikon Eclipse E100 (Nikon, Japan), and the fluorescence intensity reflected the level of m

Hydrogen Deuterium Exchange (HDX) mass spectrometry (MS) In order to prepare peptide mapping samples and undeuterated controls, both ApoE3/4 WT and ApoE3/4 M were diluted with H 2 O buffer (10 mM Tris-HCl, 50 mM NaCl, pH 7.5) to a final concentration of 2 M

metabolic changes