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Quantification of IL-6, IL-8, IL-1 and PGE2 by enzyme-linked immunoassay (ELISA) Supernatants obtained from the qPCR assay were centrifuged at 600 g for 6 min and used to estimate concentration using bovine IL-8 ELISA kits (#3114-1A-6, Mabtech, Nacka, Sweden), IL-6 (#ESS0029, Thermo Fisher Scientific), IL-1 (#ESS0027, Thermo Fisher Scientific) and PGE2 (#514010, Cayman Chemicals, Ann Arbor, MI, USA) according to the manufacturer's instructions

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Jiancheng), with the final data obtained using the microplate reader (cat

Cell lysates were then centrifuged at 20,000 g for 15 minutes at 4C and supernatants were collected

For statistical analyses, we used Graphpad Prism software v.7.02 (La Jolla, California): one-way ANOVA with Tukeys post-hoc test for cell growth assays, OCR measurements, Glutathione levels and TCA Metabolites in Figure 4e