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Twenty l of filtered MTT solution (5 mg/ml in PBS) was added to each well, after which the plates were incubated for an additional 4 h
Standards and blanks were prepared accordingly
See the Experimental Phytotherapy paragraph for more in-depth information
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Review the Gear Checklist/Recommendations to make sure you are properly prepared Have a charged cell phone with you in a drybag (or ziplock bag)
The oxidation of methionine and cysteine residues is the chief modification in crystallins associated with ARN cataract [122,123]
