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FIGURE 7 The strong role of severe oxidative stress, reduced antioxidant defenses like GSH with increased lipid peroxidation and malondialdehyde generation (213), lipid, protein and DNA oxidation with increased apoptosis and necrosis in atherosclerosis as a major cause of cardiovascular diseases and stroke, supports the use of complementary and alternative medicines, dietary supplements, and antioxidants with hardly any adverse effect, able to restore homeostasis reversing oxidative stress (214)

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doi: 10.1053/j.ajkd.2011.04.030 59 IzzedineHEderhySGoldwasserFSoriaJCMilanoGCohenAet al

doi:10.1161/CIRCULATIONAHA.115.018912

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As can be seen from the figure (Figure S13), we did not observe competitive kinetics but instead observed mixed kinetics, which indicated that juglone inhibition of ChaC1 was not competitive with glutathione as was initially expected