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The vial was left at room temperature for more than 2 hours
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It is reported that the buried tryptophan residues in folded proteins show a fluorescence emission maximum at 330335 nm whereas upon unfolding of proteins the emission maximum shifts to 350355 nm
doi: 10.1097/CCM.0000000000003555
It also measures the relative contribution of oxPPP versus non-oxPPP to ribose 5-phosphate and can be applied both in cell culture and in vivo 50,51
[DOI] [PMC free article] [PubMed] [Google Scholar] [7].Tu Y, Fan G, Wu N, Wu H, Xiao H
