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An AriaMx Real-Time PCR system was used to perform real-time polymerase chain reaction (PCR) (Agilent Technologies Applied Biosystems, Carlsbad, CA, USA)

Note: If you have a known sensitivity to sulfur or are pregnant or breastfeeding, you should consult with your healthcare provider before adding a glutathione supplement to your routine

While various risk factors for PHG have been documented, including smoking and deficiencies in vitamins (B12, folic acid, and biotin) and minerals (such as low serum calcium and ferritin), other significant factors include a family history of PHG, obesity, high blood pressure, lack of exercise, drug use, genetic syndromes, dyslipidemia, thyroid disorders, hyperuricemia, and liver function abnormalities

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In contrast with secondary bile acids, SCFA help to reinforce the colonic gut barrier through increased expression of tight junction proteins (37, 56)

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