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Modern therapeutic strategies target inflammatory mediators including TNF-, IL-17, IL-23, and intracellular pathways such as NF-B and JAK/STAT

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For solubilizing these polypeptides, variables such as non-denaturing detergents (IGEPAL CA-630), changing the ionic strength of the cell lysis and solubilization buffer, addition of BugBuster, diluting the cell lysate and sonication were introduced