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This study shows that MYC downregulation of mir-23a/b relieves repression of GLS as part of the oncogenic reprogramming of glutamine metabolism

Fryer AA, Nafee TM, Ismail KM, Carroll WD, Emes RD, Farrell WE

Subsequently, 0.4 mL of a PI staining/lysis solution (0.5% NP-40, 0.5 mM ethylenediaminetetraacetic acid [EDTA]) and 50 L of RNase A (10 mg/mL in TrisEDTA buffer, pH 8.0) solution were added

10.1161/01.hyp.32.5.902 55 LeeS.KimS

Each of these pathways involves second messengers and effector enzymes

CYP2E1, an ROS-generating enzyme of the cytochrome P450 family, is overexpressed in MASLD and contributes to both mitochondrial and microsomal ROS accumulation
