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However, this mutant still shows proteolytic processing in the recombinant protein as well upon transient expression in eukaryotic cells, suggesting processing does not use these residues for catalysis (Supplementary Figs

Processes involving air or liquid can then be used to separate the smaller, protein-rich fragments from the larger, carbohydrate-rich pieces

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Majority of the cholinesterase assays based on Ellmans reagent using the mixture of buffer and DTNB as color reagent, require freezing temperatures for storage 16,23,25