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When an addictive substance like alcohol is used chronically, more and more dopaminergic neurons are stimulated to the point where dopamine supply in the mesolimbic reward circuits can become depleted

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Probes were labeled with the VIC-reporter dye (ABI) for wild type and the FAM-reporter dye for the mutant, respectively

To activate Cre recombinase and induce Gclc deletion in tumour pieces from Gclc f/f Rosa26 creERT2 MMTV-PYMT mice, female mice were intraperitoneally injected with tamoxifen (Sigma-Aldrich, T5648) at 50160 mg kg 1 once daily for 5 consecutive days