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Henderson et al., 2009)

A clinical study reported 15 anovulatory PCOS patients aged 1831 years who completed 8-h time-restricted feeding for 6-weeks experienced substantial improvements

Cell pellets were then lysed in 50 l of 5% SDS, 100 mM TEAB and sonicated (QSonica) for 5 cycles, with a 1 min resting period on ice after each cycle