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Higher doses can stall the same process or even tip into toxicity over longer exposures, where free copper drifting loose from the complex starts driving oxidative damage

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Taught by Dutton at the EMBO course (figure 21), what Lefkovits really liked about the new technique was 'you could make an antibody without the need for a whole animal, the spleen, thymus, lymph nodes and blood.' For Lefkovits the method provided a useful starting point for studying antibody formation

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Further, animal studies indicate that it may strengthen the gut-brain axis, improving feedback between the GI tract and the central nervous system to elicit systemic effects [2, 4, 11]

Blood (1999) 93:165867