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Rac1 GTPase activates the WAVE regulatory complex through two distinct binding sites

It is plausible that GSTs serve two distinct roles in the development of drug resistance via direct detoxification as well as acting as an inhibitor of the MAP kinase pathway

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When needed, immunoprecipitation was performed with lysates of transfected cells by Flag-specific antibody-coated agarose beads (Sigma, cat# F2426) or GFP-specific antibody, which was coupled to protein G beads (Cytiva, cat# 17061801) at 4 C for 8 h