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Biochemical analysis Effect of CNPs on oxidant/antioxidant status Malondialdehyde (MDA), a lipid peroxidation marker, total antioxidant capacity (TAC), and reduced glutathione (GSH) levels were measured in brain tissue homogenate using Bio-Diagnostics colorimetric kits (CAT

The relative copy numbers of mitochondrial to nuclear DNA were determined by real-time PCR with primers specific to cytochrome c oxidase subunit II (COX II) (mitochondrial) 5-ATCCCAGGCCGACTAAATCA (forward) and 5-TTTCAGAGCATTGGCCATAGAA (reverse) and -actin (nuclear) genes 5-TATCCACCTTCCAGCAGATGT (forward) and 5-AGCTCAGTAACAGTCCGCCTA (reverse)

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While all genes discussed were derived from genetic screens in GBM, their molecular relevance to cancer may have been determined in a different tumor type

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