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RNA was isolated from liver and cell samples using TRIzol reagent (Life Technologies, Thermo Fisher Scientific, Waltham, MA, United States) and converted to cDNA using a reverse transcription kit from Thermo Fisher Scientific

For example, patients with large-artery atherosclerotic stroke and transient ischemic attack displayed obvious intestinal dysbacteriosis and reduced blood TMAO levels (Yin et al., 2015)

Vitamin B12 does so by facilitating cellular metabolism in red blood and nerve cells

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This number is right on par with the Endocrine Societys recommendation

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