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Cells were grown in M199 medium supplemented with 2% bovine growth serum (Thermo Fisher Scientific), 100 U/ml penicillinstreptomycin, and 2 mM L-glutamine

Both the AddaS03 and AS01 metE vaccine formulations induced significantly elevated IL-17 production compared to the nave group, as detected by ICS and ELISpot assays ( Figures 6A, B )

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Aliquoting reconstituted material helps minimize freeze-thaw cycles, which is one of the most reliable ways to preserve activity across a multi-week study