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doi: 10.3389/fmed.2019.00167 88 VillarroyaFCereijoRGavalda-NavarroAVillarroyaJGiraltM

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For cells treated with ML226, a final concentration of 2.5 M was maintained throughout the assay to prevent washout

The method is generally employed to estimate the sample concentration of GSH plus GSSG, i.e., total GSH (tGSH), while addition of a SH masking agent to the sample allows for accurate and precise estimation of GSSG even at the very low levels

Keeping the working area clean builds confidence