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After culturing for 48 h, pancreatic cancer cells in the upper wall were removed, and the pancreatic cancer cells on the lower surface were fixed with 4% paraformaldehyde and stained with 1% crystal violet

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Moreover, emerging evidence indicates that malnutrition-induced alterations in the gut microbiota may compromise the gut-brain axis, further exacerbating neuroinflammatory responses and influencing neuronal excitability[415]