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Cells were washed with blocking buffer 3 times for 15 min each and further incubated 2 h at RT with an appropriate secondary antibody

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Cells were lysed and GSTP1 immunoprecipitated with agarose beads conjugated to mouse anti-V5 antibody (V5-10), subjected to click chemistry, resolved on 11% SDS-PAGE and transferred to a PVDF membrane

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However, in contrast to WT parasites, SOD-B1/sodb1 deficient parasites are declined in number over time in macrophages

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