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The cells were diluted and cultured with fresh methionine-containing SD medium to the logarithmic phase

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Microbial liberation of N-methylserotonin from orange fiber in gnotobiotic mice and humans

[DOI] [PubMed] [Google Scholar] 171.Lee S.Y., Chiu Y.J., Yang S.M., Chen C.M., Huang C.C., Lee-Chen G.J., Lin W., Chang K.H

Neurons were incubated with 1:1000 dibenzocyclooctyne (DBCO) conjugated to AlexaFluor647 (#CLK-1302, Jena Bioscience, Germany) diluted in Ca 2+ /Mg 2+ -free Tyrodes solution with 1 mM EGTA (#67-42-5

To determine whether the loss of recoverable PFUs was due to bacteria being killed or due to the presence of chained bacteria that cannot form plaques (as we observed in epithelial cells), we analyzed infected BMDMs with immunofluorescence microscopy