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In a retrospective study, this model was able to accurately predict whether the overdose would lead to fatal liver damage

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The formation of superoxide anion radical leads to a cascade of other ROS

Primary antibodies used were rabbit anti-HO-1 (1:1000), mouse anti-NQO-1 (1:1000), mouse anti-p62/SQSTM1 (1:1000) and mouse anti-actin (1:5000) (see antibody characterization below and in Supplementary Table S1)

At a normal therapeutic dose, acetaminophen is primarily metabolized by the liver to sulfate and glucuronide conjugates, while a SMALL amount is metabolized by CYP2E1 to a highly reactive intermediate, N-acetyl-p-benzoquinone imine (NAPQI)