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Primary antibodies used were rabbit anti-HO-1 (1:1000), mouse anti-NQO-1 (1:1000), mouse anti-p62/SQSTM1 (1:1000) and mouse anti-actin (1:5000) (see antibody characterization below and in Supplementary Table S1)

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At a normal therapeutic dose, acetaminophen is primarily metabolized by the liver to sulfate and glucuronide conjugates, while a SMALL amount is metabolized by CYP2E1 to a highly reactive intermediate, N-acetyl-p-benzoquinone imine (NAPQI)

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