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Assessment and medication application The analgesia test was performed on the mice receiving morphine prior to the tested agents (i), and on the mice receiving no morphine prior to tested agents (ii), using the hot plate (Ugo Basille, Italy)

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ExoFlo in 100 ccs normal saline intravenously with a synchronous injection of 2 ccs ExoFlo directly into tissue around the facial nerve with ultrasound guidance

Separate application times or buffering agents can mitigate this