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It plays a significant role in cellular processes such as metabolism, DNA repair, and chromatin remodeling, which are vital for maintaining tissue and metabolic homeostasis

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Chemicals Unless otherwise indicated, the chemicals, culture medium, and supplements used in this study were purchased from Sigma-Aldrich (Shanghai, China) and Thermo Fisher (Beijing, China)

If no issues appear after two weeks, transition to daily application

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MOTS-c's AMPK activation aligns with the body's metabolic circadian rhythm, which peaks in the morning and early afternoon