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Cell numbers were determined with an Invitrogen Countess 3 Automated Cell Counter using a hemocytometer and 0.1% trypan blue (Thermo Fisher Scientific 15250061) to exclude dead cells

Most prominently, studies on RTD caused by SLC52A2/SLC52A3 mutations show that systemic delivery of AAV9-SLC52A2 in loss-of-function mouse models restores transporter expression, corrects spinal motor neuron energy deficits, reverses motor dysfunction, and extends survival

Mol Biol Rep 50 , 96499661 (2023)

They concluded that as astrocytes are a main supplier of GSH to microglia and neurons in the brain, depletion of GSH during aging or neurodegeneration in neurological diseases may not only lead to activation of microglia and the astrocytes themselves, but may also render neurons sensitive to cell death

Trends Pharmacol Sci (2018) 39(5):51324

[DOI] [PubMed] [Google Scholar] Tsutsumi H