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The integrated PK-PD findings, including nonlinear exposure, target tissue accumulation, and disruption of L-carnitine homeostasis, collectively elucidate mildronates mechanism of action through energy substrate depletion, as well as its intrinsic efficacy-toxicity duality

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In human fibroblast cultures, this has been shown to extend the "Hayflick limit" (the number of times a cell can divide) by over 10 passages

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