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Using the MTT assay (a standard test for cell viability), they measured whether AHK-Cu made these cells multiply faster

Subjects/methods Between January 2018 and December 2020, a total of 418 T2DM patients with or without insomnia were recruited

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PMID: 28121423 | doi:10.1021/acs.biochem.6b01215 Akar S et al

Published research on 5-Amino-1MQ originated with NNMT-inhibitor screens reported in the mid-2010s