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After three rinses in PBS (10 min each), the tissues were incubated with secondary antibodies in PBS containing 5% BSA at room temperature for 2 h, washed three times with PBS, mounted using Mowiol mounting medium, and imaged under a fluorescence microscope

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Available from: [DOI] [PubMed] [Google Scholar] 147.Wei Q, Duan J, Ma G, Zhang W, Wang Q, Hu Z

SCFAs are known to signal through Gproteincoupled receptors (such as GPR41 and GPR43) and histone deacetylase inhibition, pathways that play important roles in maintaining mucosal homeostasis [212, 219]