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The collected cells were suspended in 300 L ice-cold TE buffer (100 mM TrisHCl (pH 8.0), 2 mM ethylenediaminetetraacetic acid) and disrupted by a cell disruptor (Shake Master NEO, Bio Medical Sciences, Tokyo, Japan) with zirconia beads at 1500 rpm for 10 min

Cannalire G, Conti L, Celoni M, Grassi C, Cella A, Bensi G, Capelli P, Biasucci G

7.2 Regulation of B cell function The intestinal environment is where early B cell growth takes place, and bone marrow immune tolerance mechanisms help make self-reactive B cells tolerant, suggesting that the gastrointestinal tract plays a role in maintaining self-tolerance

doi: 10.1007/s10753-015-0264-4

[DOI] [PubMed] [Google Scholar] 385.Chang, Y

Zhang C, Peng Q, Tang Y, Wang C, Wang S, Yu D, Hou S, Wang Y, Zhang L, Lin N