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Briefly, the animals were synchronized by hypochlorite bleaching, hatched overnight in M9 (3 g/l KH2PO4, 6 g/l Na2HPO4, 5 g/l NaCl, 1 M MgSO4) buffer, and subsequently cultured at 20 C on nematode growth medium (NGM) (CaCl2 1mM, MgSO4 1 mM, cholesterol 5 g/ml, 250 M KH2PO4 pH 6, Agar 17 g/l, NaCl 3g/l, casein 7.5g/l) plates seeded with the E

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Your provider should evaluate whether combination therapy offers additional benefit over single-agent escalation, or whether genetic and biomarker insights reveal you may respond very well to tirzepatide monotherapy at lower overall cost and complexity

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