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Experimental and human metabolic literature demonstrates that GH promotes fatty acid mobilization, shifts substrate preference toward fat oxidation, and alters carbohydrate-lipid partitioning during fasting and metabolic stress states, making GH secretagogue systems useful tools for investigating adipose tissue signaling, fuel selection, and the endocrine regulation of energy metabolism [8]

time 24 (n) 22 (n) Rahimi (SB RCT) 8% 0.92 [0.68-1.26] hosp

Sen A, Prizant H, Hammes SR

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CRISPRCas9 method to generate KO cell lines To construct PDIA6 -KO HCT116 cells, using px330-PuroR 64 as a template, the DNA oligonucleotides 5-CGGTGTTTCGTCCTTTCCACAAGATATATA-3 and 5-AAGGACGAAACACCGACTTCTCGGTTGAAATTCGAGTTTTAGAGCTAGAAATAGCAAG-3 or 5- AAGGACGAAACACCGAACACCATACTGACCTCCTAGTTTTAGAGCTAGAAATAGCAAG-3 were utilized for inverse PCR to express guide RNAs targeting exons 2 and 4, respectively, of the PDIA6 gene