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The incidence of antibodies to semaglutide cannot be directly compared with the incidence of antibodies of other products

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doi: 10.3389/fendo.2023.1133000 Received 28 December 2022 Accepted 13 March 2023 Published 24 March 2023 Volume 14 - 2023 Edited by Silvia Martina Ferrari, University of Pisa, Italy Reviewed by Sabrina Rosaria Paparo, University of Pisa, Italy

In brief, cells were washed twice with phosphate-buffered saline and resuspended in prewarmed (37 C) Seahorse basal medium (Agilent) supplemented with 1 mM pyruvate, 2 mM glutamine, and 5 mM glucose (pH 7.4)

The 1-methylnicotinamide product of NNMT may itself have biological activities (including potential insulin resistance-promoting effects), and reducing its production through NNMT inhibition may contribute to metabolic improvements