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5.2 Challenges of clinical translation of tumor microbes Tumor-microbe interactions research is experiencing a paradigm shift from correlation to causation, the core of which lies in systematically resolving the molecular mechanisms of key functional bacteria (e.g., Clostridium nucleatum promotes tumor metastasis through activation of the Wnt/-catenin pathway by the FadA adhesin) by means of multi-omics integration technologies (e.g., single-cell sequencing, spatial transcriptomics), and utilizing synthetic biology (e.g., bionanobacterial encapsulation, CRISPRi dynamic regulation) to enhance the colonization efficiency and therapeutic controllability of engineered bacteria in the tumor microenvironment (Li et al., 2023)

As proposed by Braak and colleagues, this pattern involves six stages, starting in the peripheral nervous system and gradually affecting the central nervous system in a caudal-to-rostral direction within the brain

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Biochem Pharmacol 53:11331140

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