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Cells were harvested and lysed in lysis buffer (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 10 mM imidazole, and 2 mM PMSF) using a Pulse 150 Ultrasonic Homogenizer with 6 probe (Benchmark Scientific, Sayreville, NJ)

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Retatrutide, a GIP, GLP-1 and glucagon receptor agonist, for people with obesity

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Metabolomics is currently viewed as an extremely strong innovation to guarantee the unprejudiced and by large assurance of a few metabolites (i.e., small metabolites with a mass reach from 50 to 1500 Da)