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Jun 15 2017;13(6):813-824

The replenishment of the Krebs cycle intermediate pool through pathways independent of acetyl-CoA is termed anaplerosis

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Additionally, during the acridine orange (AO) reaction for a lysosome membrane permeability assay, the cells with indicated treatments were stained with 2 g/mL AO (Invitrogen) for 15 min at 37 C