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Chowdhury et al., 2017
CD8 + T cells were isolated from mouse spleen using EasySep Mouse Naive CD8 + T Cell Isolation Kit (STEMCELL, #19858), and cultured in plate-immobilized anti-CD3 and anti-CD28 in RPMI 1640 medium supplemented with 10% FBS, 100 U/ml penicillin & 100 g/ml streptomycin (Thermo Fisher Scientific, #15070063), 5 ng/ml IL-2 (Biolegend, #575402), 10 mM HEPES (Thermo Fisher Scientific, #15630106), 0.05 mM 2-mercaptoethanol (Thermo Fisher Scientific, #21985023) for 4 days
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La durata totale dell'effetto varia da 612 ore, con significativa variabilit inter-individuale
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If the CK level normalizes after a period of rest, further evaluation is unnecessary
