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LEC were pulsed overnight with BODIPY-PAL in replete conditions, washed, and incubated in the absence of BODIPY-PAL with Nile Red (to stain LDs) or MitoTracker deep red (to stain the mitochondria network) for 30 min (0 time) or chased further for 6 and 24 h

While it is found in foods such as red meat, dairy products, avocados, and some fish, dietary intake is often not sufficient to meet higher metabolic demands, especially for athletes or individuals following vegetarian or vegan diets

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Therefore, the production of ATP from fatty acid oxidation rather than low-yield anaerobic pathways or protein breakdown preserves body mass, muscle mass and muscle function under stress conditions [34, 35]