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After centrifugation at 10,000 g for 1 min at room temperature, the supernatant was transferred to a fresh 1.5 mL reaction tube and the protein extracts were stored at 20 C
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After another 4 h of incubation, removed the turbid medium and added 150 L DMSO to each well to dissolved the formazan crystals
That is why it is important to monitor supplementation of this element [59]
Each group consisted of 10 mice
