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Samples were thawed at room temperature and cytokine concentrations (IL-2, IL-4, IL-6, IL-10, IL-17A, IFN, TNF, soluble Fas, soluble FasL, granzyme A, granzyme B, perforin and granulysin) was quantified using the LEGENDplex Human CD8/NK V02 Panel (BioLegend, 741187), according to the manufacturers instructions

While they dont all say for certain that you have a Vitamin B12 deficiency, they are good indicators of it

doi: 10.1007/s00467-011-1928-4

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Therefore, it can not be excluded that the existence of the disulfide bridge may contribute to the formation of tetramer, while no such disulfide bridge is present in rhGPx3 mutant