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2, normal astrocytic PKM2 expression is essential for maintaining astrocyte energy supply and neuronal excitability

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Cas9, guided by sgRNAs targeting Acsl4 (5-TGAAATGCAGCCAAATGGAA-3) or Fsp1 (5-TGCACGTGGTGATCGTGGGC-3) [44], mediated cleavage of these genes

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The following steps represent standard research peptide reconstitution practice
