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(349) Taylor, R

6f, g), but did not alter LDs turnover (Fig

Permeant fluorescent probes visualize the activation of SARM1 and uncover an anti-neurodegenerative drug candidate

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After centrifuging the plates for 5 minutes at 1,500 rpm, duplicate wells were harvested and lysed with 350 l of RLT buffer

Common problems in GST tag protein purification Non-binding or weak binding of GST tag proteins Possible reasons for inefficient elution of GST tag proteins High level of heteroproteins in purified product Conclusion Generally speaking, purity of GST tag proteins via one-step affinity chromatography will be higher than that of His tag protein via one-step purification
