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I/R describes a cycle of restriction of blood (and oxygen) supply to tissues and subsequent return of the same

MTHFR also plays a central role in the transformation of folate to S-adenosylmethionine (SAM), the universal methyl donor in cells and affects DNA methylation status

Glutaminase activity is present in many tissues such as the liver, kidney, small intestine, adipose tissue, and neurons

These amino acids are then transported into the cell for the next step [89]

Folic acid in total egg folate content represented at most 10%, a level which would be converted into biologically active folates by humans after ingestion

Many studies remain at the stage of small-sample clinical trials or observational studies, and there is a lack of rigorous large-scale RCTs to validate their efficacy