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Since ASK1 is a MAPK kinase that activates the JNK and p38 pathways, GSTP1-mediated indirect inhibition of ASK1 results in cells being spared from apoptosis (Figure 5) [66]

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0.3 ml aliquots of the supernatant, 0.75 ml of 150 mM phosphate buffer (pH 7.4) containing 5 mM EDTA, and 0.15 ml of 10 mM DTT were added to determine total ascorbate

Fluorescence ( ex = 405 and 488 nm, em = 510 nm) was measured by flow cytometry using a Becton Dickinsons FACS Aria II flow cytometer