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Description
In mammalian cells, stress conditions lead to the upregulation of CHAC1 through the action of ATF4

[DOI] [PubMed] [Google Scholar] Chiang N
In the first step, GPx is oxidized by hydroperoxides

Cells were harvested and centrifuged at 1200 rpm for 3 min, followed by incubation with 1 L annexin V-Alexa Fluor 488 (640906, BioLegend) in 100 L annexin V binding buffer 1 (10 mM HEPES (pH 7.4), 0.14 M sodium chloride (NaCl), 2.5 mM calcium chloride (CaCl 2 ) and incubated at room temperature in the dark for 15 min

The levels of OEA and PEA were expressed in pmol/mg creatinine, and AEA was expressed in fmol/mg creatinine
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