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SDS-PAGE and Western Blot analysis Twenty L of sample containing 5 mg of purified rhGPx3 mutant was mixed with 20 L of loading buffer (20 mM Tris-HCl, 8% SDS, 10% -mercaptoethanol, 30% glycerol and 0.004% bromophenol blue at pH 6.8) and heated in boiling water for 5 min
10.1042/BST20140135 74

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Activation of AP-1 signal transduction pathway by SARS coronavirus nucleocapsid protein
Hyejin Ham (GIST Department of Chemistry) for technical assistance with the cellular experiments
Early diagnosis not only reverses symptoms but also prevents long-term complications such as nerve damage