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The authors show that the haemopexin domain of MMP3, and not its proteolytic activity, is essential for mammary epithelial cells invasion

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dynamic range of approximately 56 orders of magnitude , depending on sample complexity and instrument type

Following the isolation of FACS-sorted cells, both Sielaff et al., and Alfredsson et al., carried out a wash step with PBS before lysis, which was effective at removing most unwanted contaminants, such as residual BSA (Bovine Serum Albumin) and EDTA (Ethylenediaminetetraacetic acid) present in the FACS buffer 62,63

doi: 10.1016/j.semcdb.2015.07.006

Sharps container (optional) Remove the caps Sanitize the rubber stoppers Attach the needle Draw the bac water Pull back on the plunger to draw your desired volume of bacteriostatic water