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For the PV3-SC8 GPP3+GSH and PV3-SC8 pleconaril+GSH complexes, PV3-SC8 VLP and GPP3 or pleconaril were mixed at a ratio of 1 VLP:300 compound molecules, respectively, and incubated overnight at 4 C, after which GSH was added to a final concentration of 10 mM and incubated on ice for 12 h