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Modification sites were characterized on human glutathione S-transferase (hGST) alpha 1 (OLZ-modified at Cys112), hGST mu 2 (OLZ at Cys115), and hGST pi (CLZ, DCLZ, CLZ-NO and OLZ at Cys170), human microsomal GST 1 (hMGST1, CLZ and OLZ at Cys50), and human serum albumin (hSA, CLZ at Cys34)

The study also explores the AITC and protein-protein interaction among pepsin and mustard seed proteins, cruciferin and napin, as mustard contains abundant protein content but remains bio-unavailable due to presence of similar anti-nutritional factors like glucosinolates potentially having insect deterring potential

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