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Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche

doi: 10.1182/blood-2006-07-033662

However, upon NMN treatment, there were significant improvements in the overall architecture of mitochondria and they had a more intact inner membrane with visible cristae structures

Cell 142 , 914 (2010)

Consideration of the H 2 O 2 levels in unstressed aerobic cells (50 nM) and in OxyR-activated stressed cells (1 M) argues that millimolar H 2 O 2 concentrations are hugely excessive and will detect oxidation events that are vanishingly rare in nature

RNA was reverse transcribed using Moloney murine leukemia virus reverse transcriptase (Ambion- ThermoFisher Scientific) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies)
